|
|
|
| Registros recuperados: 100 | |
|
| |
|
|
| Chavez-calvillo, G.; Perez-rueda, E.; Lizama, G.; Zuniga Aguilar, J. J.; Gaxiola, G.; Cuzon, Gerard; Arena-ortiz, L.. |
| We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded... |
| Tipo: Text |
Palavras-chave: Differential display; RT-PCR; Litopenaeus vannamei; Protein diet; Alternative protein sources. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11298/7853.pdf |
| |
|
|
| Gonçalves,J.O.; Tannuri,A.C.A.; Coelho,M.C.M.; Bendit,I.; Tannuri,U.. |
| We previously described a selective bile duct ligation model to elucidate the process of hepatic fibrogenesis in children with biliary atresia or intrahepatic biliary stenosis. Using this model, we identified changes in the expression of alpha smooth muscle actin (α-SMA) both in the obstructed parenchyma and in the hepatic parenchyma adjacent to the obstruction. However, the expression profiles of desmin and TGF-β1, molecules known to be involved in hepatic fibrogenesis, were unchanged when analyzed by semiquantitative polymerase chain reaction (RT-PCR). Thus, the molecular mechanisms involved in the modulation of liver fibrosis in this experimental model are not fully understood. This study aimed to evaluate the molecular changes in an experimental model... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alpha smooth muscle actin; Desmin; Hepatic fibrogenesis; Selective bile duct ligation; RT-PCR; TGF-β1. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014001000850 |
| |
|
|
| Flores-Mendoza,Lilian; Silva-Campa,Erika; Reséndiz,Mónica; Mata-Haro,Verónica; Osorio,Fernando A.; Hernández,Jesús. |
| Las células dendríticas (DC) son las presentadoras de antígeno más importantes del sistema inmune. Su localización anatómica (piel, mucosas y sangre periférica), la expresión de receptores para reconocer patógenos, la expresión de moléculas de coestimulación (CD80/86), del complejo principal de histocompatibilidad (MHC) clases I y II, y la producción de citocinas (IFN-α, IL-10, IL-12), les confiere una característica única para regular las respuestas inmune innata y adaptativa. El objetivo de este trabajo fue evaluar el efecto del virus de síndrome reproductivo y respiratorio porcino (PRRS) en DC maduras. Se generaron células dendríticas a partir de monocitos utilizando IL-4 y GM-CSF y se estimularon con lipopolisacárido (LPS) para inducir su maduración.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Células dendríticas; Cerdo; CD80; CD86; MHC-II; RT-PCR. |
| Ano: 2009 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922009000100005 |
| |
|
|
| Michell,BC; Gomes,AD; Baião,NC; Resende,M; Lara,LJC; Martins,NRS. |
| The interference of low or high maternal antibodies titers on the attenuated infectious bursal disease (IBD) virus (IBDV) vaccine infection and its effects on the performance of broilers vaccinated at the 18th day of incubation (in ovo), at one day of age (subcutaneously-SC), or at 15 days of age (drinking water-DW) were investigated. After a series of three live vaccinations, breeders were given or not an IBD oil emulsion vaccine (IBD-OEV) prior to sexual maturity. At day 18 of incubation (in ovo), a commercial vaccine containing HVT and an intermediate IBDV strain or the single HVT vaccine was given. An intermediate IBDV vaccine was given SC at one day of age, or at 15 days of age via DW. The progeny of unvaccinated breeders presented higher neutralizing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Attenuated IBDV; Attenuated IBDV neutralization; Broiler chicken; Infectious bursal disease; Infectious bursal disease virus; IBDV; In-ovo vaccination; Maternal antibody; RT-PCR; Serum neutralization; Vaccination versus performance. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000100009 |
| |
|
|
| Soler,Maritza C; Molina,Jessica L; Díaz,Hugo A; Pinto,Vivian C; Barrios,Yasenka L; He,Kan; Roller,Marc; Weinstein-Oppenheimer,Caroline R. |
| Cimicifuga foetida, an Asian Cimicifuga species, has been employed as a cooling and detoxification agent in traditional Chinese medicine since ancient times. For this herb, two cycloartane triterpene glycosides isolated from the rhizomes have demonstrated cytotoxicity on rat tumor and human cancer cell lines. Since human Hsp27 is increased in various human cancers and exhibits cytoprotective activity that affects tumorigenesis and the susceptibility of tumours to cancer treatment, the purpose of this research was to study the expression of this protein in MCF-7 breast cancer cells. To accomplish this aim, MCF-7 cells were exposed to different concentrations of Cimicifuga foetida extract showing a reduction in cell number measured by the sulforhodamine... |
| Tipo: Journal article |
Palavras-chave: Cimicifuga foetida; Cytotoxicity; Hsp-27; Immunofluorescence; MCF-7 cells; RT-PCR. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300005 |
| |
|
| |
|
|
| Díaz,Acela; Quiñones,Madelaine; Hernández,Annia; del Barrio,Gloria. |
| El género Capsicum (Solanaceae), es originario del continente americano y comprende alrededor de 25 especies, de las cuales cinco son cultivables. Las condiciones ambientales en que se desarrolla esta hortaliza, su forma de explotación, así como los tipos y variedades utilizadas, han traído consigo una amplia diversidad en cuanto a plagas y epifitias que causan importantes mermas en su producción. Entre ellas, las enfermedades virales constituyen la principal dificultad para el desarrollo del cultivo y de estas, las causadas por los miembros del género Potyvirus, se consideran entre las de mayor importancia económica. Este trabajo tiene como objetivo validar un ensayo de RT-PCR para el diagnóstico molecular de potyvirus, empleando cebadores genéricos. Los... |
| Tipo: Journal article |
Palavras-chave: Potyvirus; Pimiento; Diagnóstico; RT-PCR. |
| Ano: 2010 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522010000200002 |
| |
|
| |
|
| |
|
|
| CONTRERAS NIETO,ELÍZABETH; LÓPEZ CARRASCAL,CAMILO E. |
| La yuca (Manihot esculenta Crantz, Euphorbiaceae) es el cuarto cultivo en importancia a nivel mundial como fuente de calorías para la población humana y cuya producción se ve afectada por la bacteriosis vascular, enfermedad ocasionada por Xanthomonas axonopodis pv. manihotis (Xam). La resistencia a enfermedades en plantas depende de la presencia de genes de resistencia (R), los cuales reconocen a los patógenos y simultáneamente permiten desencadenar la respuesta de defensa. A pesar de recientes esfuerzos encaminados a la identificación de genes R en yuca, aún no se ha logrado clonar el primer gen R en este cultivo. En el presente trabajo se estudió el perfil de expresión de dos Genes Candidatos a Resistencia (RGCs) asociados a QTLs de defensa contra la... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Xam; RGC; RT-PCR. |
| Ano: 2008 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2008000200012 |
| |
|
|
| Batista,J.J.; Martins,A.S.; Moro,L.; Vasconcelos,A.C.. |
| Determinou-se a expressão gênica das caspases 3 e 8 mediante transcrição reversa de mRNA total e reação em cadeia da polimerase (RT-PCR) para avaliar a apoptose em timo e baço de ratas imunossuprimidas por glicocorticóides. Utilizou-se dexametasona para indução da apoptose e atrofia linfóide. Quarenta e cinco fêmeas Wistar recém-desmamadas foram separadas em três grupos: as ratas de A (n=18) e B (n=18) foram tratadas com 250 e 500mg de glicocorticóide, via intramuscular, respectivamente, e as do C (n=9) não foram tratadas. Após 24, 48 e 72 horas, seis animais de cada grupo tratado e três do controle foram anestesiados, pesados e sacrificados. O baço e o timo foram coletados e pesados. Fragmentos dos órgãos foram fixados em formol tamponado a 10% e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rato; Apoptose; Dexametasona; Timo; Baço; Caspase 3; Caspase 8; RT-PCR. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000400006 |
| |
|
|
| Faleiro,Mariana Batista Rodrigues; Cintra,Lorena Cardoso; Jesuino,Rosália Santos Amorim; Araújo,Eugênio Gonçalves de; Rocha,Rafael Malagoli; Moura,Veridiana Maria Brianezi Dignani de. |
| ABSTRACT: Gene expression of ErbB1 and ErbB2, and immunostaining of EGFR (Her1) and Her2 (c-erbB-2) were evaluated in this study to ascertain whether these receptors are involved in the evolution of canine premalignant and malignant prostatic lesions, as proliferative inflammatory atrophy (PIA) and prostatic carcinoma (PC). With regards to the intensity of EGFR immunostaining, there was no difference between normal prostatic tissue and tissues with PIA or PC. In relation to Her2 immunostaining, there were differences between normal prostatic tissue and those with PIA and PC, as also differences between prostates with PIA and PC. There was no correlation between EGFR and Her2 immunostaining. ErbB1 gene product was detected in two normal tissue samples, in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Growth factor receptor; Her1; C-erbB-2; PIA; RT-PCR. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017001200553 |
| |
|
|
| Yildirim,Aysegul Burcin; Karabulut,Derya; Dundar,Munis; Ulusoy,Hasan Basri; Sonmez,Mehmet Fatih. |
| The aim of this work was to study the relative ghrelin and growth hormone secretagogue receptor (GHS-R)1a gene expression in the kidney of long-term diabetic rats. Forty male Wistar albino rats were divided into four groups: C- control group, DI- one month diabetic rats group, DII- two months diabetic rats group, and DIII- three months diabetic rats group. Diabetes was induced by streptozotocin STZ (40mg/kg i.p). The rats were decapitated under ketamine anesthesia and their kidney tissues were removed. Tissue GHS-R mRNA levels, ghrelin expression, and histopathological damage scores were compared. Dilatation in the distal tubules, epithelial desquamation into the lumen of the tubules and transparent tubules showing glycogen vacuolation were observed in all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes mellitus; Ghrelin; GHSR-1a; Kidney; Immunohistochemistry; RT-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100300 |
| |
|
|
| Silveira,Hebert; Melo,Antonio Diego Brandão; Bortoluzzi,Cristiano; Costa,Leandro Batista; Rostagno,Marcos Horácio; Schinckel,Allan Paul; Garbossa,Cesar Augusto Pospissil; Cantarelli,Vinícius de Souza. |
| ABSTRACT The intestinal gene expression of RelA/p65 (NF-κB), insulin growth factor 1 (IGF-1), glucose transporter 2 (GLUT2), and Na+/dependent glucose transporter 1 (SGLT1) were evaluated in response to benzoic acid, yeast culture, L-glutamine, and oregano essential oil, using an ex vivo model. Six piglets weighing approximately 20 kg each were sacrificed, and their jejunum was collected and segmented into five 2-cm explants. Each explant was immersed in cell culture medium according to one of the following treatments: control (without additive), 0.5% benzoic acid, 1% yeast culture, 1% L-glutamine, and 0.015% oregano oil. Gene expression was evaluated using RT-PCR. Yeast culture up-regulated the gene expression of RelA/p65, IGF-1, GLUT2, and SGLT1 in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chemosensors; Glucose; Piglet; RT-PCR; Swine; Trophic effects. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982018000100414 |
| |
|
|
| Merino,J.O.; Cruz,NI. De la; Galvan,G.; León,A.P. De; Burnes,J.. |
| ABSTRACT Epizootic hemorrhagic disease viruses (EHDV) are dsRNA arboviruses transmitted by biting midges of the genus Culicoides that cause disease in domestic and wild ruminants. Epizootic hemorrhagic disease (EHD) is considered the most important infectious disease of white tailed deer (WTD) in North America, some studies in Northeast Mexico reported EHDV-seropositive WTD and EHDV-infected Culicoides vectors. The increasing population of WTD that share habitat with livestock in Northeast México highlights the importance of EHD for the livestock industry in the transboundary region with the U.S. One hundred and twenty two samples from WTD in Tamaulipas state, Mexico were tested by ELISA and RT-PCR for EHDV antibodies and nucleic acid, respectively. Twelve... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Deer; Detection; EHDV; RT-PCR; Serology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100077 |
| |
|
| |
|
|
| Chatel, Annelle; Faucet-marquis, Virginie; Gourlay-france, Catherine; Pfohl-leszkowicz, Annie; Hubert, Francoise. |
| The aim of the present study was to confirm the relevance of studying DNA adduct formation in a field study. In that context, freshwater mussels Dreissena polymorpha, collected in a reference station, were transplanted in different sites with a pollution gradient. After one and two months, mussels were collected and DNA adduct formation was analyzed using the 32P post labelling technique on both gills and digestive glands. In addition, the expression of genes involved in the detoxification system (catalase (CAT), superoxide dismutase (SOD), glutathione S-transferase (GST), HSP70, aryl hydrocarbon receptor (AHR), P glycoprotein (PgP), metallothionein (MT)) was assessed by RT-PCR. DNA adducts were observed at amount comparable to data from literature.... |
| Tipo: Text |
Palavras-chave: Biomarker; Pollution; DNA adducts; Detoxification; Dreissena polymorpha; RT-PCR. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00195/30639/32450.pdf |
| |
|
|
| Teixeira,J.M.S.; Camara,G.N.N.L.; Pimentel,P.F.V.; Ferreira,M.N.R.; Ferreira,M.S.R.; Alfieri,A.A.; Gentsch,J.R.; Leite,J.P.G.. |
| Group C rotaviruses are fastidious in their in vitro cell culture requirements. Recent serosurveys indicate that antibody to group C rotavirus is present in 3-45% of the human population in certain geographic locations, suggesting that rotavirus group C infection is more prevalent than previously believed and that the low rate of detection of these agents is probably due to the lack of sensitive diagnostic assays. From March to December 1994, 406 fecal specimens were collected from children under five years of age who were outpatients at the emergency services of nine public hospitals in Brasília, Federal District, Brazil. In addition to the samples from children, one public outpatient unit requested virological investigation of a stool sample from an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Group C rotavirus; Diarrhea; HIV; RT-PCR; Probe hybridization. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001100005 |
| |
|
|
| Bosso,Patrícia Alves Ramos; Candeias,João Manuel Grisi; Paduan,Karina dos Santos; Ricchetti,Sandra Mara Queiroz; Miranda,Antero Frederico Macedo de; Rugolo,Lígia Maria Suppo de Souza; Durigon,Edison Luiz; Ventura,Armando Morais. |
| Human Respiratory Syncytial Virus (HRSV) is recognized as the single most important pathogen responsible for acute viral infection of the lower respiratory tract in young children. In this study, 84 samples from children under two years of age presenting acute respiratory symptoms were obtained from September 2000 to November 2001. Analysis by immunofluorescence and reverse-transcription followed by PCR, revealed that 18% (15/84) of all the samples were positive for HRSV, in which 80% (12/15) of the cases was observed in children under six months of age and the subgroups A and B co-circulated in the studied period. These are the first data obtained for Botucatu city and seasonality is evident by the higher circulation of the virus between May and July. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human Respiratory Syncytial Virus (HRSV); RT-PCR; Immunofluorescence; Seasonality. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300015 |
| |
| Registros recuperados: 100 | |
|
|
|
